2020
Ikeda-Ohtsubo W, López Nadal A, Zaccaria E, Iha M, Kitazawa H, Kleerebezem M and Brugman S. Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus). Front. Nutr. 7:67 (2020)
doi: 10.3389/fnut.2020.00067
オランダ・ワーゲニンゲン大学とコラボでゼブラフィッシュモデルを用いてオキナワモズクに含まれるフコイダンの自然免疫と腸内フローラの調節作用を明らかにした論文
Villena J* and Kitazawa H*. The Modulation of Mucosal Antiviral Immunity by Immunobiotics: Could They Offer Any Benefit in the SARS-CoV-2 Pandemic? Front. Physiol., 11:699 (2020) doi: 10.3389/fphys.2020.00699
アルゼンチン国立乳酸菌研究所とコラボのイムノバイオティクスによる呼吸器系の抗ウイルス免疫調節作用と新型コロナウイルス（SARS-CoV-2）への応用可能性をまとめた総説
Fukuyama K, Islam Md A, Takagi M, Ikeda-Ohtsubo W, Kurata S, Aso H, Vignolo G, Villena J, Kitazawa H*. Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay. Pathogens, 9:410 (2020)
(FK, IMdA and TM have contributed equally to this work)
アルゼンチン国立乳酸菌研究所、CFAI畜産免疫ユニットとコラボでウシ乳腺上皮細胞を用いた乳牛由来乳酸菌の乳房炎に対する免疫調節機能評価系を確立した論文
Mizuno H, Arce L, Tomotsune K, Albarracin L, Funabashi R, Daniela Vera, Islam Md A, Vizoso-Pinto MG, Takahashi H, Sasaki Y, Kitazawa H*, Villena J*. Lipoteichoic acid is involved in the ability of the immunobiotic strain Lactobacillus plantarum CRL1506 to modulate the intestinal antiviral innate immunity triggered by TLR3 activation. Front. Immunol., 11:571 (2020) doi: 10.3389/fimmu.2020.00571
(MH and AL have contributed equally to this work)
アルゼンチン国立乳酸菌研究所、明治大学とコラボでイムノバイオティクスLactobacillus plantarum CRL1506の腸管抗ウイルス自然免疫の調節作用にリポテイコ酸が関与していることを明らかにした論文
Albarracin L, García-Castillo V, Masumizu Y, Indo Y, Islam Md A, Suda Y, Garcia A, Aso H, Takahashi H, Kitazawa H*, Villena J*. Efficient selection of new immunobiotic strains with antiviral effects in local and distal mucosal sites by using porcine intestinal epitheliocytes. Front. Immunol., 11:543 (2020) doi: 10.3389/fimmu.2020.00543
(AL and GCV have contributed equally to this work)
当研究室に留学していたアルゼンチン国立乳酸菌研究所のLeoさんによる、CFAI畜産免疫ユニット、宮城大学とコラボでブタ腸管上皮細胞を用いた抗ウイルス作用を有する新規イムノバイオティクスの効率的選抜法を確立した研究の論文
García-Castillo V, Marcial G, Albarracin L, Tomokiyo M, Clua P, Takahashi H, Kitazawa H*, Garcia-Cancino A*, Villena J*. The exopolysaccharide of Lactobacillus fermentum UCO-979C is partially involved in its immunomodulatory effect and its ability to improve the resistance against Helicobacter pylori infection. Microorganisms, 8:479 (2020)
doi: 10.3390/microorganisms8040479
当研究室に留学していたチリ・コンセプシオン大学Valeriaさんによる、アルゼンチン国立乳酸菌研究所とのコラボでプロバイオティクス乳酸菌Lactobacillus fermentum UCO-979Cの細胞外多糖の免疫調節性とピロリ菌耐性の改善効果を明らかにした論文
Ortiz Moyano R, Raya Tonetti F, Tomokiyo M, Kanmani P, Vizoso-Pinto MG, Kim H, Quilodrán-Vega S, Melnikov V, Alvarez S, Takahashi H, Kurata S, Kitazawa H*, Villena J*. The Ability of Respiratory Commensal Bacteria to Beneficially Modulate the Lung Innate Immune Response Is a Strain Dependent Characteristic. Microorganisms, 8:727 (2020)
doi: 10.3390/microorganisms8050727
(OMR, RTF, and TM have contributed equally to this work)
López Nadal A, Ikeda-Ohtsubo W, Sipkema D, Peggs D, McGurk C, Forlenza M, Wiegertjes GF, Brugman S. Feed, Microbiota, and Gut Immunity: Using the Zebrafish Model to Understand Fish Health. Front. Immunol., 11:114 (2020) doi: 10.3389/fimmu.2020.00114
オランダ・ワーゲニンゲン大学とコラボの「ゼブラフィッシュモデルを用いた飼料・腸内フローラ・腸管免疫の研究」の総説
Islam Md A, Takagi M, Fukuyama K, Komatsu R, Albarracin L, Nochi T, Suda Y, Ikeda-Ohtsubo W, Rutten V, van Eden W, Villena J, Aso H*, Kitazawa H*. Transcriptome analysis of the inflammatory responses of bovine mammary epithelial cells: exploring immunomodulatory target genes for bovine mastitis. Pathogens, 9:200 (2020)
doi: 10.3390/pathogens9030200
(IMdA, TM, and FK have contributed equally to this work)
オランダ・ユトレヒト大学、アルゼンチン国立乳酸菌研究所、CFAI畜産免疫ユニット、宮城大学とコラボでウシ腸管上皮細胞の炎症応答に伴って発現が変化する（乳房炎治療のターゲット候補となる）遺伝子を明らかにした論文。
Xu R, Karrow NA*, Shandilya UK, Sun L-h, Kitazawa H. In-vitro cell culture for efficient assessment of mycotoxin exposure, toxicity and risk mitigation. Toxins, 12:146 (2020) doi: 10.3390/toxins12030146
カナダ・Guelph大学とコラボの腸管上皮細胞を用いたマイコトキシン（カビ毒）評価系の総説
Tada A, Islam Md A, Kober AKMH, Fukuyama K, Takagi M, Igata M, Albarracin L, Ikeda-Ohtsubo W, Miyazawa K, Yoda K, He F, Takahashi H, Villena J, Aso H*, Kitazawa H*. Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin. Int. J. Mol. Sci., 21:638 (2020) doi: 10.3390/ijms21020638
(TA, KAKMH, and IMdA have contributed equally to this work)
CFAIの作物免疫ユニット、畜産免疫ユニット、アルゼンチン国立乳酸菌研究所、タカナシ乳業とコラボで「TNF-αおよびセロトニンによる分化および外因性刺激時のブタ筋肉内脂肪細胞のトランスクリプトーム修飾」を明らかにした研究の論文
Raya Tonetti F, Arce L, Salva S, Alvarez S, Takahashi H, Kitazawa H, Vizoso-Pinto MG, Villena J. Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants. Front. Immunol., 11:15 (2020) doi: 10.3389/fimmu.2020.00015
CFAIの作物免疫ユニット、アルゼンチン国立乳酸菌研究所とコラボの乳酸菌イムノバイオティクスから得られた細菌様粒子（BLP）の免疫調節作用とアジュバントへの応用可能性に関する研究の論文
Raya Tonetti F, Islam MA, Vizoso-Pinto MG, Takahashi H, Kitazawa H*, Villena J*. Nasal priming with immunobiotic lactobacilli improves the adaptive immune response against influenza virus. Int. Immunopharmacol., 78:106115 (2020)
doi: 10.1016/j.intimp.2019.106115
2019
Takahashi H, Fukuhara T, Kitazawa H, Kormelink R. Virus latency and the impact on plants. Front. Microbiol. 10:2764 1-18 (2019) doi:10.3389/fmicb.2019.02764
Garcia-Castillo V, Marín-Vega AM, Ilabaca A, Albarracin L, Marcial G, Kitazawa H, Garcia-Cancino A, Villena J. Characterization of the immunomodulatory and anti-Helicobacter pylori properties of the human gastric isolate Lactobacillus rhamnosus UCO-25A. Biofouling, 35(8):922-937 (2019) doi: 10.1080/08927014.2019.1675153
Iida H, Tohno M, Islam Md A, Sato N, Kobayashi H, Albarracin L, Kober AKMH, Ikeda-Ohtsubo W, Suda Y, Aso H, Nochi T, Miyazaki A, Uenishi H, Iwabuchi N, Xiao J-Z, Villena J*, Kitazawa H*. Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells. Cells 8:891 (2019) doi: 10.3390/cells8080891
(IH, TM, and IMdA have contributed equally to this work)
Garcia-Castillo V, Komatsu R, Clua P, Indo Y, Takagi M, Salva S, Islam Md A, Alvarez S, Takahashi H, Garcia-Cancino A*, Kitazawa H*, Villena J*. Evaluation of the immunomodulatory activities of the probiotic strain Lactobacillus fermentum UCO-979C. Front. Immunol. 10:1376 (2019) doi: 10.3389/fimmu.2019.01376
Masumizu Y, Zhou B, Kober AKMH, Islam Md A, Iida H, Ikeda-Ohtsubo W, Suda Y, Albarracin L, Nochi T, Aso H, Suzuki K, Villena J, Kitazawa H*. Isolation and Immunocharacterization of Lactobacillus salivarius from the Intestine of Wakame-Fed Pigs to Develop Novel “Immunosynbiotics”. Microorganisms 7:167 (2019) doi: 10.3390/microorganisms7060167
(MY, ZB, KAKMH, and IMdA have contributed equally to this work)
Igata M, Islam Md A, Tada A, Takagi M, Kober AKMH, Albarracin L, Aso A, Ikeda-Ohtsubo W, Miyazawa K, Yoda K, He F, Takahashi H, Villena J*, Kitazawa H*. Transcriptome modifications in porcine adipocytes via toll-like receptors activation. Front. Immunol. 10:1180 (2019) doi: 10.3389/fimmu.2019.01180
(IM and IMdA have contributed equally to this work)
Silvina Fadda, Julio Villena, Leonardo Albarracin, Lucila Saavedra, Md. Aminul Islam, Graciela M. Vignolo, Haruki Kitazawa, Elvira Maria Hebert. Draft Genome Sequence of Lactobacillus plantarum CRL681, Isolated from Argentinean Artisanal Fermented Sausages. Microbiol. Res. Announc. 8(15) e01629-18 (2019)
Garcia-Cancino A, Albarracin A, Espinoza-Monje M, Campos-Martin J, Garcia-Castillo V, Nakano Y, Ikeda-Ohtsubo W, Guitierrez-Zamorano C, Morita H, Kitazawa H*, Villena H*, Draft Genome Sequence of Weissella viridescens UCO-SMC3, Isolated from the Slime of Helix aspersa Müller Snails. Microbiol. Res. Announc. 8(11) e01654-18 (2019)
Oyetibo GO, Miyauchi K, Huang Y, Ikeda-Ohtsubo W, Chien MF, Ilori MO, Amund OO, Endo G. Comparative geochemical evaluation of toxic metals pollution and bacterial communities of industrial effluent tributary and a receiving estuary in Nigeria. Chemosphere 227:638-646 (2019) doi: 10.1016/j.chemosphere.2019.04.048
2018
How can we define“optimal microbiota”? : a comparative review of structure and functions of microbiota of animals, fish, and plants in agriculture.[Front. Nut.,in press]Wakako Ikeda-Ohtsubo*, Sylvia Brugman, Craig H Warden, Johanna M. J. Rebel, Gert Folkerts and Corné M.J. Pieterse
Receptors and signaling pathways for recognition of bacteria in livestock and crops: prospects for beneficial microbes in healthy growth strategies.[Front. Immunol.,in press]Julio Villena*, Haruki Kitazawa, Saskia C M Van Wees, Corné M.J. Pieterse and Hideki Takahashi
A Comparative Review on Microbiota Manipulation: Lessons From Fish, Plants, Livestock, and Human Research.[Front. Nut.,in press]
Sylvia Brugman*, Wakako Ikeda-Ohtsubo, Saskia Braber, Gert Folkerts , Corné M. J. Pieterse and Peter A. H. M. Bakker
Genomic characterization of Lactobacillus delbrueckii TUA4408L and evaluation of the antiviral activities of its extracellular polysaccharides in porcine intestinal epithelial cells.[Front. Immunol.,in press]Paulraj Kanmani , Leonardo Albarracin , Hisakazu Kobayashi, Elvira Maria Hebert , Lucila Saavedra, Ryoya Komatsu, Brian Gatica, Alexis Salas Burgos, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso , Shintaro Egusa, Takashi Mishima, Hideki Takahashi , Julio Villena* and Haruki Kitazawa
Immunobiotics for the Bovine Host: Their Interaction with Intestinal Epithelial Cells and Their Effect on Antiviral Immunity.[Front. Immunol.,in press]Julio Villena*, Hisashi Aso , Victor P. M. G. Rutten , Hideki Takahashi , Willem van Eden and Haruki Kitazawa*
Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells.[Mol. Immunol.,93,(2018),253-265]Kanmani, P, L. Albarracin, H. Kobayashi, H. Iida, R. Komatsu, AKM H. Kober, W. Ikeda-Ohtsubo, Y. Suda, H. Aso, S. Makino, H. Kano, T. Saito, J. Villena*, H. Kitazawa*
2017
Draft genome sequence of immunobiotic Lactobacillus rhamnosus IBL027 a potential adjuvant for mucosal vaccine development.[Genome Announc.,5(50),(2017),e01268-17-]María Guadalupe Vizoso-Pinto, M. G., L. Saavedra, E. Hebert, F. R. Tonetti, L. Albarracin, S. Alvarez, H. Kitazawa*, J. Villena*
Editorial: Immunobiotics - Interactions of Beneficial Microbes with the Immune System.[Front. Immunol.,8(1580),(2017),1-2]Villena, J.*, H. Kitazawa*
Peptidoglycan from immunobiotic Lactobacillus rhamnosus improves resistance of infant mice to respiratory syncytial viral infection and secondary pneumococcal pneumonia.[Front. Immunol.,8(948),(2017),1-15]Clua, P., P. Kanmani, H. Zelaya, A. Tada, AKM H. Kober, S. Salva, S. Alvarez, H. Kitazawa*, J. Villena*
Respiratory commensal bacteria Corynebacterium pseudodiphtheriticum improves resistance of infant mice to Respiratory Syncytial Virus and Streptococcus pneumoniae superinfection.[Front. Microbiol.,8(1613),(2017),1-14]Kanmani, P.†, P. Clua†, M. G. Vizoso-Pinto, C. Rodriguez, S. Alvarez, V. Melnikov, H. Takahashi, H. Kitazawa*, J. Villena* (†: Equal contribution)
Effects of mycoplasmal pneumonia of swine (MPS) lung lesion-selected Landrace pigs on MPS resistance and immune competence in three-way crossbred pigs.[Anim. Sci. J.,88(4),(2017),575-585]Borjigin, L., T. Shimazu, Y. Katayama, K. Watanabe, H. Kitazawa, S-g. Roh, H. Aso, K. Kazuo, M. Satoh, Y. Suda, A. Sakuma, M. Nakajo, K. Suzuki*
Probiotic Microorganisms: A Closer Look.[Microorganisms,5(2),(2017),1-3]Villena, J*., H. Kitazawa*
Transcriptomic analysis of the innate antiviral immune response in porcine intestinal epithelial cells: influence of immunobiotic lactobacilli.[Front. Immunol.,8(57),(2017),1-15]Albarracin, L.†, H. Kobayashi†, H. Iida, N. Sato, T. Nochi, H. Aso, S. Salva, S. Alvarez, H. Kitazawa*, J. Villena* (†: Equal contribution)
Draft genome sequence of the immunobiotic strain Lactobacillus jensenii TL2937.[Genome Announc.,5(9),(2017),e00005-17-]Villena, J.*, Y. Masumizu, H. Iida, W. Ikeda-Ohtsubo, L. Albarracin, S. Makino, S. Ohkawara, K. Kimura, L. Saavedra, E. Hebert, H. Kitazawa*
Development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes.[Beneficial Microbes,8(2),(2017),309-321]Kobayashi, H.#, P. Kanmani#, T. Ishizuka, A. Miyazaki, J. Soma, L. Albarracin, Y. Suda, T. Nochi, H. Aso, N. Iwabuchi, J-z. Xiao, T. Saito, J. Villena*, H. Kitazawa* (# Equal contribution)
Draft genome sequence of Lactobacillus plantarum MPL16 a wakame-utilizing immunobiotic strain from swine feces.[Genome Announc.,5(10),(2017),e00006-17-]Villena, J.*, L. Saavedra, E. Hebert, Y. Suda, Y. Masumizu, L. Albarracin, P. Clua, W. Ikeda-Ohtsubo, H. Kitazawa*
Development of immune and microbial environments is independently regulated in the mammary gland.[Mucosal Immunol.,(2017)]Niimi, K. K. Usami, Y. Fujita, M. Abe, M. Furukawa, Y. Suyama, Y. Sakai, M. Kamioka, N. Shibata, E.J. Park, S. Sato, H. Kiyono, H. Yoneyama, H. Kitazawa, K. Watanabe, T. Nochi*, H. As